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ĥؼNM360 20*2000*80000 5500 NM400 20*2200*8000 6500 NM400 20*2200*10000 6500 дĥۼ۸Żݻӭ磺021-56692669 13917985004 021-36070335 13701664517С

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dz̸ʳƷ΢о ʹ󳦾Ⱥⶨע۶


ʱ䣺2019-10-01  

۶ľժҪʳƷȫѾΪϵȺڽش⣬ҹʳƷȫϾ˹ҵĸ߶ӣʳƷ΢ȾʳƷϸǰСľʳƷ΢ѧгĿʹ󳦾ȺⶨӦעз֣̽ϣͬ

ķչǶʳҪҲԽԽߣ“Եú”ѾΪҵձ󣬶ʳƷȫ“Եú”ǰᡣȻʳƷҵӹδϸҪ߰װϴλ˹̲Ʒ΢ָ곬꣬Ӷʹʳ߽ܵΣ

ǰҹʳƷȫල΢ĿҪо󳦾Ⱥùͽĸ²4ࡣӳʳƷϸȾij̶ȣ󳦾ȺӳʳƷǷбȾùͽĸʹʳƷܱʣΣ彡²ʳжУʹ󳦾ȺĿΪѧʳƷҪָ꣬Ǿ󲿷ʳƷıؼĿ2019ϰгֹܾIJϸʳƷУʹ󳦾Ⱥռ΢ﳬֱΪ60.78%11.76%[[[] . 2019ϰгֹܾϸʳƷ256ΣߴҵҪע [N]. й, 2019-06-28(6).]]ˣΪȺڵĽȫ߾ʹ󳦾Ⱥ׼ȷԡ߽ϹʵʣʳƷоʹ󳦾ȺⶨӦעвϣͬ

ѡȷļ鷽Ǽ׼ȷɿǰᡣĿǰʳƷоⶨķҪGB 4789.2-2016ˮⶨȴΪGB/T 5750.12-2006׼IJͬѡõGB 4789.2-2016 Ϊƽ֬PCAGB/T 5750.12-2006 ΪӪ֬NAߵƽ֬ϸĻָͬʱֹܷϸƬ״[2] , 뵤, ܻ, . ͬŨ TTC ƽ֬ϸ۲[J]. й־, 2012, 22(1): 62-63GB 4789.2-2016ⶨˮоܵ²ⶨƫ߶ʹƷС

ڴ󳦾Ⱥ⣬еĹұ׼ΪGB 4789.3-2016ñ׼ַMPNƽ巨MPNһְ붨ڴ󳦾Ⱥϵ͵ʳƷļ⣻ƽ巨Ϊڴ󳦾ȺϸߵʳƷļ⡣ȷʹַĹؼֲƷִб׼޶ȵĵλ޶Ҫ“CFU/ g CFU/mL”ΪλʱӦѡƽ巨“MPN/g MPN/mL”ΪλģӦѡMPNȻһЩIJƷ罴͡׺һЩʳƷ޶Ҫ“MPN/100mLMPN /100g”ΪλʱӦѡGB/T 4789.3-2003м⣬Ϊ 2009 16 Ź ȷ 涨ʳƷ׼й涨Ĵ󳦾Ⱥָ“MPN/100˻MPN/100”ΪλģáʳƷ΢ѧ󳦾ȺⶨGB/T4789.3-2003м⡣ӦƷѡȷļ鷽

ʹ󳦾ȺIJⶨӦڽྻУ߾ϸĿǰ΢ʵҳõʽҪΪʪࡣиú¸160桫180ĸȣɱϸѿߣﵽĿĵһַʪøѹԼдڵDZúõĴ͸ʹ嵰̱Զʹ΢һַһ㲣󡢿̶Լȡ߿װӦIJͰУȻȿ160~1802h߹ЧʣƻٷӦ̵ »ˮƷˮʪķ.

ƷȡǰһҪ˲ƷװܷԺܷԡԱҪϸչ涨޾ñӡ ֡ ׵ȣǰþƾֲȡʱӦע޾һƷ䲻ͬλȡ25g225mL޾ˮУҺƷȡ25mL225mL޾ˮСӦעǣԲͬʳƷƷǰĹ涨ʳ׺ϣڳԣpHֵԲܽм⣻ζƷ罴ͽ͵ȣ䱾ϸߣȡϡƷʱӦ޾ˮ̬̬ƷȡʱӦ޾ˮԤ45 棻䶳ʳƷȡǰӦ 45² 15 min , 2桫 5治 18 h нⶳ

ʹ󳦾Ⱥ鳣õϡҺΪ޾ˮλҺһ߿ͨãκȻһЩԻƷʹλҺΪϡҺƷ΢Ĵͼ[[[] , . ʹòͬϡҺʳƷоӰ̽[J]. , 2012, 39(1):86-88.]]Ʊ10ϵϡƷҺʱӦƷȾ̶ȣԼƷ΢޶ѡϡ͵ͱҺ̬Ͼ޶Ҫn=5, c=2, m=100CFU/mL, M=10000CFU/mLʱϡ͵1100ͿжϲƷǷϸ񣻶涨n=5, c=2, m=10000 CFU/g, M=100000 CFU/gʱҪϡ͵11000ⶨܽжϣäⶨʱڲ֪ƷʱΪӦϡͶȣһϡ͵10-8ܴﵽĿġϡ͹Уÿϡһһ޾ܻͷ֤ϡͱ׼ȷԣͬʱӦȡհϡҺԶգ Ϊհ׶ϸ˵ϡҺԱһδʵҪ󣬼ЧӦһŲԭ

ھʱӦýҿIJʹҺӲ߽롣Һ󣬱뾡עȡעӦ46棬Ϊ¶ȹеϸܱӰ׼ȷԣ¶ȹ̡̺֬󣬽361浹48h2hˮƷΪ30172h3hԭҪ31.ֹʱˮγˮȾ2.֬治ˮĤӶʹγɣ3.ٶˣγɵľ׼

ڴ󳦾ȺУƽ巨ע;ⶨƣҲע¶ȱ46棬ʹMPNⶨǰҪעϿ۹ǷпӰĹ۲죬ӹڽʱӦע⣬Ʒ1mLһΪ10mLʱӦʹ˫1mLӦõΪϡʹɷŨȽͣʹ˫

ڴ󳦾ȺMPNǷʵУ۲ǷӦԹб۲죬ʱϿ۹лֱСݸżԱʱͨ۲췢ҺǷǣǷжϡGB/T 4789.3-2003ͨ۲췢ҺǷƽжϣȷǷһʵ顣Ϊ֤ʵ׼ȷԣӦͬʱԶաԶ顣Ʒʱ֤ʵ飬ΪоʵԵԹܣ֤ʵΪԡ

ڴ󳦾Ⱥƽ巨֤Уɾȡ֤ǼԱΪȽϼֵ⡣涨ȡ10ͬ͵ĵͺͿɾ䣬10ȫȡ仰üԱ֪⡣ʵĵ;Ҫ󳦰ϣϾ˾ײϾ˾ԱʵʱȡϸԶ飬Ȼע۲죬¼ʹ󳦾Ⱥֳ̬;Ϳɾ䡣ʽʱȡ;Ϳɾ10ȫȡ֤ȡʱͬԷ©

ʹ󳦾Ⱥļһ㰴չ涨ִмɡȻʵʼ鹤г޷߽Ͻļ龭ΪԴ¼⡣ȣǾǴ󳦾Ⱥⶨ̺֬渲3~4mLӦֹӣΣԶϡͶȣڸϡͱٴΣӦÿ۲죬ھǰ¼ģھⶨ£Գƽ֬TTCȻĵ򣩣TTCԭǴ󲿷ϸڴлĹܲøãɽɫTTCԭʹԺɫڼͬʱƴƬӾγ[4,5]ʹùӦϸTTCŨȣӰ

ھʹ󳦾Ⱥⶨʱʱ޷ֲ΢;ΡʱӦϸ۲죬ѰҲһ״򣬶Թ򣻲ɫ⻹ͨӳʱжϣΪΪϸͨھⶨͨһƽ壬ע֬ŵ0-4УʱΪգTTCʹϸȾɺɫм

⣬ھʹ󳦾Ⱥʱָܳϡͱϸϡͱ10ϵϡͱϸڵϡͱǼ̲ƷлзʱļӦΪݡ

ھʹ󳦾ȺⶨʱӰⶨһΪؼأӦ÷GB 4789.28-2013׼ҪһЩõƽ֬LST¹ȵǵη͹ܣһβȫ꣬4䣬һɱܡƽֻ֬ڻһʹãڻӰʵ󳦾Ⱥƽ巨УVRBAᾧԺ쵨֬Ӧʹǰ3СʱƱԷܵȾ

ʳƷȫǵĽϢϢأϰʳƷȫҪָʾҪ“Ͻı׼ϸļܡĴ”ΪһʳƷ΢ԱʳƷ⼼淢չĽ죬ӦʱռԭԼעҪ㡣ھʹ󳦾ȺʱҪÿһϸ׼ȷܱ֤׼ȷɿΪҹʳƷȫܹ׳

[1] . 2019ϰгֹܾϸʳƷ256ΣߴҵҪע [N]. й

[2], 뵤, ܻ, . ͬŨ TTC ƽ֬ϸ۲[J]. й־

[3], . ʹòͬϡҺʳƷоӰ̽[J].

[4]. TTCʳƷ΢ⶨеӦ÷[J]. ҽѧϢժ

[5] Ф, , ¿, . ʳƷ΢֤Ʒ鷽[J]. ʳƷȫѧ

1.ŽʳƷ, Ž3320002.ŽʳƷҩƷ, Ž332000

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